Undetected location. NO YES. Handbook of Derivatives for Chromatography, 2nd Edition. Selected type: Hardcover.
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Added to Your Shopping Cart. This is a dummy description. A practical handbook of derivatives and an invaluable reference source for chemists using chromatography for derivatization.
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Since publication of the first edition in , there have been many developments in chemical derivatization which have gone hand-in-hand with advances in chromatography. All of these reagents carry at least one aromatic ring or even larger pi-electron systems, which are responsi-ble for the UV detection.
Blau K., Halket J.M. Handbook of Derivatives for Chromatography s
Dabsylchloride can, for example, be used to derivatize amines, thiols, imidazoles, phenols and alcohols. Example for alcohols: Mix 1 mL of alcohol with 10 mL mM sodium bicarbonate and 20 mL 10 mM dabsyl chloride, reflux for 10 minutes, cool, extract with diethylether, wash with pH 3 citrate buffer, evaporate the organic layer, reconstitute and inject an aliquot.
Chemical reagents called fluorotags are used to introduce groups with fluorescent properties into the molecules.
Table 2 shows a list of common fluorotag reagents:. In-ject an aliquot of the hexane layer. This is about acylation reactions with perfluorinated organic acid anhydrides, like trifluoroacetic acid anhy-dride or the homologous pentafluoropropanoic and heptafluorobutanoic acid anhydrides.
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Acylation reactions are described in part one of this document , which was about gas chromatography. Post-column derivatizations are online chemical reactions, carried out after the separation has taken place and before the component enters the detector:.
Handbook of derivatives for chromatography, second edition
An example for the method used in Figure 4 is the post-column fluorotag derivatization of sulfonamides :. Figure 5: Post-column derivatization of Sulfanilamide blue with benzene-1,2-dicarbaldehyde OPA and 2-sulfanylethanol. The exitation can be measured at nm and the emission at nm wavelengths. Another possibility for post-column reactions are immobilized enzyme reactors IMERs , where an enzyme is used to catalyze certain reactions before the component goes to the detector.
The first reaction 1 is catalyzed by acetylcholine esterase and the second 2 by choline oxidase, two enzymes immobilized in the IMER reactor.