Guide Handbook of derivatives for chromatography

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Undetected location. NO YES. Handbook of Derivatives for Chromatography, 2nd Edition. Selected type: Hardcover.

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Added to Your Shopping Cart. This is a dummy description. A practical handbook of derivatives and an invaluable reference source for chemists using chromatography for derivatization.

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Since publication of the first edition in , there have been many developments in chemical derivatization which have gone hand-in-hand with advances in chromatography. All of these reagents carry at least one aromatic ring or even larger pi-electron systems, which are responsi-ble for the UV detection.

Blau K., Halket J.M. Handbook of Derivatives for Chromatography s

Dabsylchloride can, for example, be used to derivatize amines, thiols, imidazoles, phenols and alcohols. Example for alcohols: Mix 1 mL of alcohol with 10 mL mM sodium bicarbonate and 20 mL 10 mM dabsyl chloride, reflux for 10 minutes, cool, extract with diethylether, wash with pH 3 citrate buffer, evaporate the organic layer, reconstitute and inject an aliquot.

Chemical reagents called fluorotags are used to introduce groups with fluorescent properties into the molecules.

Derivative of Logarithmic Functions

Table 2 shows a list of common fluorotag reagents:. In-ject an aliquot of the hexane layer. This is about acylation reactions with perfluorinated organic acid anhydrides, like trifluoroacetic acid anhy-dride or the homologous pentafluoropropanoic and heptafluorobutanoic acid anhydrides.

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  7. Acylation reactions are described in part one of this document , which was about gas chromatography. Post-column derivatizations are online chemical reactions, carried out after the separation has taken place and before the component enters the detector:.

    Handbook of derivatives for chromatography, second edition

    An example for the method used in Figure 4 is the post-column fluorotag derivatization of sulfonamides [1]:. Figure 5: Post-column derivatization of Sulfanilamide blue with benzene-1,2-dicarbaldehyde OPA and 2-sulfanylethanol. The exitation can be measured at nm and the emission at nm wavelengths. Another possibility for post-column reactions are immobilized enzyme reactors IMERs , where an enzyme is used to catalyze certain reactions before the component goes to the detector.


    The first reaction 1 is catalyzed by acetylcholine esterase and the second 2 by choline oxidase, two enzymes immobilized in the IMER reactor.